Please consider donating today. Your contributions help ensure the BCIWiki continues to grow as an open informational resource. Click here to learn more.
Also check out this Letter to the Neurotech Community.
EASI-FISH
Determining the spatial organization and morphological characteristics of molecularly defined cell types is a major bottleneck for characterizing the architecture underpinning brain function. Expansion-Assisted Iterative Fluorescence In Situ Hybridization (EASI-FISH) was developed to survey gene expression in brain tissue, as well as a turnkey computational pipeline to rapidly process large EASI-FISH image datasets. EASI-FISH was optimized for thick brain sections (300 µm) to facilitate reconstruction of spatio-molecular domains that generalize across brains.