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Pynapse is a coding interface for controlling event-driven behavior and performing closed-loop studies. Pynapse has a Python-based programming environment with built-in autocomplete to assist with coding. For behavior paradigms, Pynapse controls behavior inputs and outputs, such as lever presses, nose pokes, and pellet dispensers, and drives event-based behavioral states. Pynapse can also process data from our Synapse software online, such as calculating neural firing rates and averaging evoked responses.